Primer

Part:BBa_K4998006:Design

Designed by: Markos Mathioudakis   Group: iGEM23_thessaloniki   (2023-10-06)

HetR gene left primer


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

From the software's proposed primers we chose the one with the longest product size so that we can observe a band in the agarose GEL after the PCR.

Source

We designed this part using the Primer3 software.

References

https://primer3.ut.ee/